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KMID : 1377020140110040284
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Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 4 p.284 ~ p.290
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Nanocomposite bioactive polymeric scaffold promotes adhesion, proliferation and osteogenesis of rat bone marrow stromal cells
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Jin Guang-Zhen
Kim Hae-Won
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Abstract
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Synthetic degradable polymer scaffolds have often shown poor cell affinity and bone bioactivity. Bioactive inorganic nanoadditives with the composition of calcium silicate in amorphous phase (CSN) were incorporated within poly (lactic acid) (PLA) to develop nanocomposite bone scaffolds. Nanocomposite solutions containing PLA with 10% CSN were porous-structured by a salt-impregnation / leaching method. The nanocomposite scaffold showed higher dynamical mechanical modulus values than the pure PLA equivalent, indicating a proper usage for hard tissue scaffolds. Rat bone marrow stromal cells (rBMSCs) were cultured on the scaffolds and the cell adhesion, proliferation and osteogenic differentiation at gene and protein levels were addressed. Cells adhered better on the nanocomposite scaffold with respect to PLA equivalent, and then proliferated more during the culture periods of up to 7 days. The expression of osteogenic genes, including collagen type I, osteopontin, osteocalcin and alkaline phosphatase, were significantly stimulated on the nanocomposite scaffold, as confirmed by a real time polymerase chain reaction. Furthermore, Western blot analysis indicated higher expression of osteopontin protein of cells by the nanocomposite scaffold. Results demonstrated the bioactive nanocomposite scaffolds provided appropriate matrix conditions for rBMSCs to populate on and to undergo osteogenic processes in vitro.
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KEYWORD
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Nanocomposites, bone scaffolds, nanoadditives, bone marrow stromal cells, osteogenic differentiation
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